Control of mutation frequency by bacteriophage T4 DNA polymerase. II. Accuracy of nucleotide selection by the L88 mutator, CB120 antimutator, and wild type phage T4 DNA polymerases.
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چکیده
منابع مشابه
Control of mutation frequency by bacteriophage T4 DNA polymerase. I. The CB120 antimutator DNA polymerase is defective in strand displacement.
The ts CB1200 (antimutator) mutation in bacteriophage T4 DNA polymerase increases the accuracy of DNA replication since it results in a decrease in the frequency of mutations in other phage genes. The CB120 polymerases differs from the wild type enzyme in the slow rate at which it copies templates where primer extension requries displacement of polynucleotides base-paired to the template strand...
متن کاملRegulation of DNA polymerase exonucleolytic proofreading activity: studies of bacteriophage T4 "antimutator" DNA polymerases.
DNA polymerases replicate DNA with high fidelity lab, which demonstrated that single point mutations in the T4 DNA polymerase gene can increase or decrease because of accurate nucleotide incorporation coupled with exonucleolytic proofreading to remove misinmutation rates by 100-fold or more (Drake et al. 1969). Mutational analysis is a powerful method to probe corporated nucleotides. This state...
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DNA polymerases achieve high-fidelity DNA replication in part by checking the accuracy of each nucleotide that is incorporated and, if a mistake is made, the incorrect nucleotide is removed before further primer extension takes place. In order to proofread, the primer-end must be separated from the template strand and transferred from the polymerase to the exonuclease active center where the ex...
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15 صفحه اولNucleotide sequence of a type II DNA topoisomerase gene. Bacteriophage T4 gene 39.
T4 DNA topoisomerase is a type II enzyme and is thought to be required for normal T4 DNA replication T4 gene 39 codes for the largest of the three subunits of T4 DNA topoisomerase. I have determined the nucleotide sequence of a region of 2568 nucleotides of T4 DNA which includes gene 39. The location of the gene was established by the identification of the first fifteen amino acids in the large...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1976
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)33150-2